› 1r7j

X-ray diffraction
1.47Å resolution

Crystal structure of the DNA-binding protein Sso10a from Sulfolobus solfataricus

Released:
DOI: 10.2210/pdb1r7j/pdb
PDB entry 1r7j coloured by chain, front view.
PDB entry 1r7j coloured by chain, side view.
PDB entry 1r7j coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
The hyperthermophile protein Sso10a is a dimer of winged helix DNA-binding domains linked by an antiparallel coiled coil rod.
Chen L, Chen LR, Zhou XE, Wang Y, Kahsai MA, Clark AT, Edmondson SP, Liu ZJ, Rose JP, Wang BC, Meehan EJ, Shriver JW
J Mol Biol 341 73-91 (2004)
PMID: 15312764

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178461 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ArnR1-like winged helix-turn-helix domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 95 amino acids
Theoretical weight: 11.1 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5W1E8 (Residues: 2-96; Coverage: 99%)
Gene name: sso10a
Sequence domains: Winged helix-turn-helix
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21212
Unit cell:
a: 57.94Å b: 72.3Å c: 30.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.244
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The interplay between nucleoid organization and transcription in archaeal genomes.
Peeters et al. (2015)
3 more

12 mentions without citation

Macromolecular ab initio phasing enforcing secondary and tertiary structure.
Millán et al. (2015)
11 more