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› 1r7y

X-ray diffraction
1.75Å resolution

Glycosyltransferase A in complex with 3-amino-acceptor analog inhibitor and uridine diphosphate

Released:
DOI: 10.2210/pdb1r7y/pdb
Model geometry
Fit model/data
PDB entry 1r7y coloured by chain, front view.
PDB entry 1r7y coloured by chain, side view.
PDB entry 1r7y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The influence of an intramolecular hydrogen bond in differential recognition of inhibitory acceptor analogs by human ABO(H) blood group A and B glycosyltransferases.
Nguyen HP, Seto NO, Cai Y, Leinala EK, Borisova SN, Palcic MM, Evans SV
J Biol Chem 278 49191-5 (2003)
PMID: 12972418

Function and Biology Details

Reactions catalysed: 
an alpha-L-fucosyl-(1->2)-beta-D-galactosyl derivative + UDP-alpha-D-galactose = an alpha-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->2)]-beta-D-galactosyl derivative + UDP + H(+).
an alpha-L-fucosyl-(1->2)-beta-D-galactosyl derivative + UDP-N-acetyl-alpha-D-galactosamine = an N-acetyl-alpha-D-galactosaminyl-(1->3)-[alpha-L-fucosyl-(1->2)]-beta-D-galactosyl derivative + UDP + H(+).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-147725 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P16442 (Residues: 64-345; Coverage: 80%)
Gene name: ABO
Sequence domains: Glycosyltransferase family 6
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

Carbohydrate polymer : NEW Components: AIG, FUC
Ligand AIG 1 x AIG
Ligand FUC 1 x FUC
3 bound ligands:
Ligand HG 5 x HG
Ligand MN 1 x MN
Ligand UDP 1 x UDP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X8C
Spacegroup: C2221
Unit cell:
a: 52.5Å b: 149.4Å c: 79.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.216
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
12 other articles