› 1r8m

X-ray diffraction
1.7Å resolution

SEC7 DOMAIN OF THE ARF EXCHANGE FACTOR ARNO WITH BREFELDIN A-SENSITIZING MUTATIONS

Released:
DOI: 10.2210/pdb1r8m/pdb
PDB entry 1r8m coloured by chain, front view.
PDB entry 1r8m coloured by chain, side view.
PDB entry 1r8m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural snapshots of the mechanism and inhibition of a guanine nucleotide exchange factor.
Renault L, Guibert B, Cherfils J
Nature 426 525-30 (2003)
PMID: 14654833

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189195 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytohesin-2 Chain: E
Molecule details ›
Chain: E
Length: 203 amino acids
Theoretical weight: 23.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99418 (Residues: 50-252; Coverage: 51%)
Gene names: ARNO, CYTH2, PSCD2, PSCD2L
Sequence domains: Sec7 domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand FMT 1 x FMT
1 modified residue:
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A, ESRF BEAMLINE ID14-3
Spacegroup: P21
Unit cell:
a: 33.429Å b: 95.775Å c: 33.665Å
α: 90° β: 101.67° γ: 90°
R-values:
R R work R free
0.194 0.192 0.231
Expression system: Escherichia coli

Quick links

Citations

45 review citations

GEFs and GAPs: critical elements in the control of small G proteins.
Bos et al. (2007)
44 more

5 mentions without citation

In silico structure-based approaches to discover protein-protein interaction-targeting drugs.
Shin et al. (2017)
4 more