› 1r8w

X-ray diffraction
2.5Å resolution

Native structure of the B12-independent glycerol dehydratase from clostridium butyricum

Released:
DOI: 10.2210/pdb1r8w/pdb
PDB entry 1r8w coloured by chain, front view.
PDB entry 1r8w coloured by chain, side view.
PDB entry 1r8w coloured by chain, top view.
Source organism: Clostridium butyricum
Primary publication:
Insight into the mechanism of the B12-independent glycerol dehydratase from Clostridium butyricum: preliminary biochemical and structural characterization.
O'Brien JR, Raynaud C, Croux C, Girbal L, Soucaille P, Lanzilotta WN
Biochemistry 43 4635-45 (2004)
PMID: 15096031

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184502 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 787 amino acids
Theoretical weight: 88.15 KDa
Source organism: Clostridium butyricum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GEZ8 (Residues: 1-787; Coverage: 100%)
Gene name: dhaB1
Sequence domains:
Structure domains: Anaerobic Ribonucleotide-triphosphate Reductase Large Chain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 103.434Å b: 212.912Å c: 199.498Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.171 0.203
Expression system: Escherichia coli

Quick links

Citations

23 review citations

The Radical SAM Superfamily.
Frey et al. (2008)
22 more

3 mentions without citation

Radical S-adenosylmethionine enzymes.
Broderick et al. (2014)
2 more