› 1r9c

X-ray diffraction
1.83Å resolution

Crystal Structure of Fosfomycin Resistance Protein FosX from Mesorhizobium Loti

Released:
DOI: 10.2210/pdb1r9c/pdb
PDB entry 1r9c coloured by chain, front view.
PDB entry 1r9c coloured by chain, side view.
PDB entry 1r9c coloured by chain, top view.
Source organism: Mesorhizobium loti
Primary publication:
Mechanistic diversity of fosfomycin resistance in pathogenic microorganisms.
Fillgrove KL, Pakhomova S, Newcomer ME, Armstrong RN
J Am Chem Soc 125 15730-1 (2003)
PMID: 14677948

Function and Biology Details

Reaction catalysed: 
Prenyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189148 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fosfomycin resistance protein FosX Chains: A, B
Molecule details ›
Chains: A, B
Length: 139 amino acids
Theoretical weight: 16.21 KDa
Source organism: Mesorhizobium loti
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q98GG1 (Residues: 1-139; Coverage: 100%)
Gene names: fosX, mlr3345
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 45.034Å b: 84.024Å c: 66.861Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.243
Expression system: Escherichia coli BL21(DE3)

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Citations

16 review citations

Challenges of antibacterial discovery.
Silver LL. (2011)
15 more

4 mentions without citation

Protein subunit interfaces: heterodimers versus homodimers.
Zhanhua et al. (2005)
3 more