› 1r9j

X-ray diffraction
2.22Å resolution

Transketolase from Leishmania mexicana

Released:
DOI: 10.2210/pdb1r9j/pdb
PDB entry 1r9j coloured by chain, front view.
PDB entry 1r9j coloured by chain, side view.
PDB entry 1r9j coloured by chain, top view.
Source organism: Leishmania mexicana mexicana
Primary publication:
Transketolase from Leishmania mexicana has a dual subcellular localization.
Veitch NJ, Maugeri DA, Cazzulo JJ, Lindqvist Y, Barrett MP
Biochem J 382 759-67 (2004)
PMID: 15149284

Function and Biology Details

Reaction catalysed: 
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185322 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transketolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 673 amino acids
Theoretical weight: 72.2 KDa
Source organism: Leishmania mexicana mexicana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8MPM3 (Residues: 1-671; Coverage: 100%)
Gene name: TKT
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TPP 2 x TPP
1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 76.8Å b: 120.44Å c: 139.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.213
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The return of metabolism: biochemistry and physiology of the pentose phosphate pathway.
Stincone et al. (2015)
3 more

5 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
4 more