› 1ra4

X-ray diffraction
1.86Å resolution

Crystal structure of the Methanococcus jannaschii L7Ae protein

Released:
DOI: 10.2210/pdb1ra4/pdb
PDB entry 1ra4 coloured by chain, front view.
PDB entry 1ra4 coloured by chain, side view.
PDB entry 1ra4 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
The crystal structure of the Methanocaldococcus jannaschii multifunctional L7Ae RNA-binding protein reveals an induced-fit interaction with the box C/D RNAs.
Suryadi J, Tran EJ, Maxwell ES, Brown BA
Biochemistry 44 9657-72 (2005)
PMID: 16008351

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156936 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large ribosomal subunit protein eL8 Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 12.99 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: P54066 (Residues: 1-117; Coverage: 100%)
Gene names: MJ1203, rpl7ae
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 43.897Å b: 46.36Å c: 53.975Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.223
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Non-coding RNAs: lessons from the small nuclear and small nucleolar RNAs.
Matera et al. (2007)
9 more

1 mention without citation

The automated optimisation of a coarse-grained force field using free energy data.
Caceres-Delpiano et al. (2021)