› 1rbj

X-ray diffraction
2.7Å resolution

RIBONUCLEASE B COMPLEX WITH D(TETRA-(DEOXY-ADENYLATE))

Released:
DOI: 10.2210/pdb1rbj/pdb
PDB entry 1rbj coloured by chain, front view.
PDB entry 1rbj coloured by chain, side view.
PDB entry 1rbj coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure of a ribonuclease B+d(pA)4 complex.
Ko TP, Williams R, McPherson A
Acta Crystallogr D Biol Crystallogr 52 160-4 (1996)
PMID: 15299737

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114509 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain
DNA (5'-D(*AP*AP*AP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 4 nucleotides
Theoretical weight: 1.21 KDa
Source organism: Bos taurus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41212
Unit cell:
a: 44.45Å b: 44.45Å c: 156.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 not available not available
Expression system: Not provided

Quick links

Citations

3 citation in other articles

Recognition of nucleic acid bases and base-pairs by hydrogen bonding to amino acid side-chains.
Cheng et al. (2003)
2 more

3 mentions without citation

Protein-RNA interactions: a structural analysis.
Jones et al. (2001)
2 more