› 1rc7

X-ray diffraction
2.15Å resolution

Crystal structure of RNase III Mutant E110K from Aquifex Aeolicus complexed with ds-RNA at 2.15 Angstrom Resolution

Released:
DOI: 10.2210/pdb1rc7/pdb
PDB entry 1rc7 coloured by chain, front view.
PDB entry 1rc7 coloured by chain, side view.
PDB entry 1rc7 coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Noncatalytic assembly of ribonuclease III with double-stranded RNA.
Blaszczyk J, Gan J, Tropea JE, Court DL, Waugh DS, Ji X
Structure 12 457-66 (2004)
PMID: 15016361

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130430 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease 3 Chain: A
Molecule details ›
Chain: A
Length: 220 amino acids
Theoretical weight: 26.02 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67082 (Residues: 1-220; Coverage: 100%)
Gene names: aq_946, rnc
Sequence domains:
Structure domains:
5'-R(*GP*GP*CP*GP*CP*GP*CP*GP*CP*C)-3' Chains: B, C, D, E
Molecule details ›
Chains: B, C, D, E
Length: 10 nucleotides
Theoretical weight: 3.21 KDa
Source organism: Aquifex aeolicus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 58.286Å b: 118.142Å c: 106.665Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.242
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

22 review citations

Dicing and slicing: the core machinery of the RNA interference pathway.
Hammond SM. (2005)
21 more

10 mentions without citation

RNA recognition by double-stranded RNA binding domains: a matter of shape and sequence.
Masliah et al. (2013)
9 more