› 1rcm

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF A UBIQUITIN-DEPENDENT DEGRADATION SUBSTRATE: A THREE-DISULFIDE FORM OF LYSOZYME

Released:
DOI: 10.2210/pdb1rcm/pdb
PDB entry 1rcm coloured by chain, front view.
PDB entry 1rcm coloured by chain, side view.
PDB entry 1rcm coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Crystal structure of a ubiquitin-dependent degradation substrate: a three-disulfide form of lysozyme.
Hill CP, Johnston NL, Cohen RE
Proc Natl Acad Sci U S A 90 4136-40 (1993)
PMID: 8387211

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Not provided
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

1 bound ligand:
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 77.7Å b: 81.3Å c: 38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 not available
Expression system: Not provided

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Citations

15 citation in other articles

A proteolytic pathway that recognizes ubiquitin as a degradation signal.
Johnson et al. (1995)
14 more

2 mentions without citation

Single disulfide bond reduced papain exists in a compact intermediate state.
Edwin et al. (2000)
1 more