› 1rds

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF RIBONUCLEASE MS (AS RIBONUCLEASE T1 HOMOLOGUE) COMPLEXED WITH A GUANYLYL-3',5'-CYTIDINE ANALOGUE

Released:
DOI: 10.2210/pdb1rds/pdb
PDB entry 1rds coloured by chain, front view.
PDB entry 1rds coloured by chain, side view.
PDB entry 1rds coloured by chain, top view.
Source organism: Aspergillus phoenicis
Primary publication:
Crystal structure of ribonuclease Ms (as a ribonuclease T1 homologue) complexed with a guanylyl-3',5'-cytidine analogue.
Nonaka T, Nakamura KT, Uesugi S, Ikehara M, Irie M, Mitsui Y
Biochemistry 32 11825-37 (1993)
PMID: 8218254

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132814 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease Ms Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 11.41 KDa
Source organism: Aspergillus phoenicis
Expression system: Not provided
UniProt:
  • Canonical: P00653 (Residues: 1-105; Coverage: 100%)
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:
Ligand GPC 1 x GPC
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 46.52Å b: 60.57Å c: 34.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 not available not available
Expression system: Not provided

Quick links

Citations

3 review citations

A decade of protein engineering on ribonuclease T1--atomic dissection of the enzyme-substrate interactions.
Steyaert J. (1997)
2 more

4 mentions without citation

LIGSITEcsc: predicting ligand binding sites using the Connolly surface and degree of conservation.
Huang et al. (2006)
3 more