› 1rep

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURE OF REPLICATION INITIATOR PROTEIN REPE54 OF MINI-F PLASMID COMPLEXED WITH AN ITERON DNA

Released:
DOI: 10.2210/pdb1rep/pdb
PDB entry 1rep coloured by chain, front view.
PDB entry 1rep coloured by chain, side view.
PDB entry 1rep coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of a prokaryotic replication initiator protein bound to DNA at 2.6 A resolution.
Komori H, Matsunaga F, Higuchi Y, Ishiai M, Wada C, Miki K
EMBO J 18 4597-607 (1999)
PMID: 10469640

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114514 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Replication initiation protein Chain: C
Molecule details ›
Chain: C
Length: 251 amino acids
Theoretical weight: 29.34 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03856 (Residues: 1-251; Coverage: 100%)
Gene names: E, ECOK12F045, rep, repE
Sequence domains:
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*GP*T)-3') Chain: A
Molecule details ›
Chain: A
Length: 22 nucleotides
Theoretical weight: 6.69 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 22 nucleotides
Theoretical weight: 6.81 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: C2
Unit cell:
a: 108.435Å b: 81.853Å c: 73.891Å
α: 90° β: 121.53° γ: 90°
R-values:
R R work R free
0.213 0.213 0.274
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Control of plasmid DNA replication by iterons: no longer paradoxical.
Chattoraj DK. (2000)
8 more

17 mentions without citation

Structural characterization of the O-GlcNAc cycling enzymes: insights into substrate recognition and catalytic mechanisms.
Joiner et al. (2019)
16 more