› 1rfq

X-ray diffraction
3Å resolution

Actin Crystal Dynamics: Structural Implications for F-actin Nucleation, Polymerization and Branching Mediated by the Anti-parallel Dimer

Released:
DOI: 10.2210/pdb1rfq/pdb
PDB entry 1rfq coloured by chain, front view.
PDB entry 1rfq coloured by chain, side view.
PDB entry 1rfq coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Actin crystal dynamics: structural implications for F-actin nucleation, polymerization, and branching mediated by the anti-parallel dimer.
Reutzel R, Yoshioka C, Govindasamy L, Yarmola EG, Agbandje-McKenna M, Bubb MR, McKenna R
J Struct Biol 146 291-301 (2004)
PMID: 15099571

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159317 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin, alpha skeletal muscle Chains: A, B
Molecule details ›
Chains: A, B
Length: 375 amino acids
Theoretical weight: 41.86 KDa
Source organism: Oryctolagus cuniculus
Expression system: Not provided
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ATP 2 x ATP
Ligand LAR 2 x LAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P43
Unit cell:
a: 101.5Å b: 101.5Å c: 104.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.261
Expression system: Not provided

Quick links

Citations

2 review citations

Nuclear actin extends, with no contraction in sight.
Pederson et al. (2005)
1 more

3 mentions without citation

Structural basis for the slow dynamics of the actin filament pointed end.
Narita et al. (2011)
2 more