› 1rgi

X-ray diffraction
3Å resolution

Crystal structure of gelsolin domains G1-G3 bound to actin

Released:
DOI: 10.2210/pdb1rgi/pdb
PDB entry 1rgi coloured by chain, front view.
PDB entry 1rgi coloured by chain, side view.
PDB entry 1rgi coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the N-terminal half of gelsolin bound to actin: roles in severing, apoptosis and FAF.
Burtnick LD, Urosev D, Irobi E, Narayan K, Robinson RC
EMBO J 23 2713-22 (2004)
PMID: 15215896

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159303 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gelsolin Chain: G
Molecule details ›
Chain: G
Length: 346 amino acids
Theoretical weight: 38.84 KDa
Source organism: Equus caballus
UniProt:
  • Canonical: Q28372 (Residues: 2-347; Coverage: 47%)
Gene name: GSN
Sequence domains: Gelsolin repeat
Structure domains: Severin
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 42.1 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 1-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3121
Unit cell:
a: 145.245Å b: 145.245Å c: 129.948Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.224 0.258

Quick links

Citations

10 review citations

Regulation of the actin cytoskeleton-plasma membrane interplay by phosphoinositides.
Saarikangas et al. (2010)
9 more

14 mentions without citation

The state of the filament.
Aguda et al. (2005)
13 more