Function and Biology Details
Biochemical function:
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
mRNA decay activator protein ZFP36L2 and RNA (preferred)
PDBe Complex ID:
PDB-CPX-155684 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
mRNA decay activator protein ZFP36L2
Molecule details ›
Chain: A
Length: 70 amino acids
Theoretical weight: 8.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Length: 70 amino acids
Theoretical weight: 8.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P47974 (Residues: 151-220; Coverage: 14%)
Sequence domains: Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Ligands and Environments
1 bound ligand:
No modified residues
Experiments and Validation Details
Refinement method:
Individual zinc finger domains were created in DYANA and docked to UAUU (finger I) and UUAUU (finger II) using five rounds of simulated annealing in AMBER using distance and torsional restraints. Finger I/UAUU and Finger II/UUAUU complexes were connected to form TZF/UUAUUUAUU complexes and refined in AMBER with residual dipolar coupling restraints added. Calculations were then switched from in vacuo to Generalized Born continuum solvent model and one additional round of simulated annealing was performed.
Expression systems:
- Escherichia coli BL21(DE3)
- Not provided
