› 1rif

X-ray diffraction
2Å resolution

Crystal structure of the UvsW helicase from Bacteriophage T4

Released:
DOI: 10.2210/pdb1rif/pdb
PDB entry 1rif coloured by chain, front view.
PDB entry 1rif coloured by chain, side view.
PDB entry 1rif coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
The crystal structure of the UvsW helicase from bacteriophage T4.
Sickmier EA, Kreuzer KN, White SW
Structure 12 583-92 (2004)
PMID: 15062081

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149040 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent DNA helicase uvsW Chains: A, B
Molecule details ›
Chains: A, B
Length: 282 amino acids
Theoretical weight: 32.85 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20703 (Residues: 1-282; Coverage: 48%)
Gene names: dar, uvsW
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand AU 4 x AU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 42.739Å b: 95.181Å c: 86.278Å
α: 90° β: 95.4° γ: 90°
R-values:
R R work R free
0.221 0.218 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Assembly and dynamics of the bacteriophage T4 homologous recombination machinery.
Liu et al. (2010)
1 more

4 mentions without citation

Understanding helicases as a means of virus control.
Frick et al. (2006)
3 more