› 1rjc

X-ray diffraction
1.4Å resolution

Crystal structure of the camelid single domain antibody cAb-Lys2 in complex with hen egg white lysozyme

Released:
DOI: 10.2210/pdb1rjc/pdb
PDB entry 1rjc coloured by chain, front view.
PDB entry 1rjc coloured by chain, side view.
PDB entry 1rjc coloured by chain, top view.
Source organisms:
Primary publication:
Strong in vivo maturation compensates for structurally restricted H3 loops in antibody repertoires.
De Genst E, Silence K, Ghahroudi MA, Decanniere K, Loris R, Kinne J, Wyns L, Muyldermans S
J Biol Chem 280 14114-21 (2005)
PMID: 15659390

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208247 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
camelid heavy chain antibody Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 14.9 KDa
Source organism: Camelus dromedarius
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Lysozyme C Chain: B
Molecule details ›
Chain: B
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 50.02Å b: 68.36Å c: 72.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.235
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Engineered antibody fragments and the rise of single domains.
Holliger et al. (2005)
8 more

8 mentions without citation

Molecular basis for the preferential cleft recognition by dromedary heavy-chain antibodies.
De Genst et al. (2006)
7 more