› 1rjm

X-ray diffraction
2.15Å resolution

Crystal Structure of MenB (Rv0548c) from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb1rjm/pdb
PDB entry 1rjm coloured by chain, front view.
PDB entry 1rjm coloured by chain, side view.
PDB entry 1rjm coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structure of naphthoate synthase (MenB) from Mycobacterium tuberculosis in both native and product-bound forms.
Johnston JM, Arcus VL, Baker EN
Acta Crystallogr D Biol Crystallogr 61 1199-206 (2005)
PMID: 16131752

Function and Biology Details

Reaction catalysed: 
4-(2-carboxyphenyl)-4-oxobutanoyl-CoA = 1,4-dihydroxy-2-naphthoyl-CoA + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1,4-dihydroxy-2-naphthoyl-CoA synthase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 339 amino acids
Theoretical weight: 37.83 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WNP5 (Residues: 1-314; Coverage: 100%)
Gene names: Rv0548c, menB
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EP1 1 x EP1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P43212
Unit cell:
a: 80.305Å b: 80.305Å c: 257.553Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.203 0.231
Expression system: Escherichia coli

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Citations

1 review citation

Advances in Key Drug Target Identification and New Drug Development for Tuberculosis.
Mi et al. (2022)
10 other articles