› 1rjw

X-ray diffraction
2.35Å resolution

CRYSTAL STRUCTURE OF NAD(+)-DEPENDENT ALCOHOL DEHYDROGENASE FROM BACILLUS STEAROTHERMOPHILUS STRAIN LLD-R

Released:
DOI: 10.2210/pdb1rjw/pdb
PDB entry 1rjw coloured by chain, front view.
PDB entry 1rjw coloured by chain, side view.
PDB entry 1rjw coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystal structure and amide H/D exchange of binary complexes of alcohol dehydrogenase from Bacillus stearothermophilus: insight into thermostability and cofactor binding.
Ceccarelli C, Liang ZX, Strickler M, Prehna G, Goldstein BM, Klinman JP, Bahnson BJ
Biochemistry 43 5266-77 (2004)
PMID: 15122892

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154611 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 339 amino acids
Theoretical weight: 36.38 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P42328 (Residues: 1-339; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand ETF 13 x ETF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 68.322Å b: 138.222Å c: 158.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.252
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Hydrogen tunneling links protein dynamics to enzyme catalysis.
Klinman et al. (2013)
4 more

16 mentions without citation

Impaired protein conformational landscapes as revealed in anomalous Arrhenius prefactors.
Nagel et al. (2011)
15 more