› 1rk0

X-ray diffraction
2.61Å resolution

Mhc Class I H-2Kb Heavy Chain Complexed With beta-2 Microglobulin and Herpes Simplex Virus Glycoprotein B peptide

Released:
DOI: 10.2210/pdb1rk0/pdb
PDB entry 1rk0 coloured by chain, front view.
PDB entry 1rk0 coloured by chain, side view.
PDB entry 1rk0 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the restoration of TCR recognition of an MHC allelic variant by peptide secondary anchor substitution.
Miley MJ, Messaoudi I, Metzner BM, Wu Y, Nikolich-Zugich J, Fremont DH
J Exp Med 200 1445-54 (2004)
PMID: 15557346

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135043 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, K-B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.65 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01901 (Residues: 22-295; Coverage: 79%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Envelope glycoprotein B Chain: P
Molecule details ›
Chain: P
Length: 8 amino acids
Theoretical weight: 923 Da
Source organism: Human alphaherpesvirus 1 strain F
Expression system: Not provided
UniProt:
  • Canonical: P06436 (Residues: 498-505; Coverage: 1%)
Gene names: UL27, gB

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 134.89Å b: 90.222Å c: 45.451Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.238
Expression systems:
  • Drosophila melanogaster
  • Not provided

Quick links

Citations

3 review citations

Low-affinity peptides and T-cell selection.
Ziegler et al. (2009)
2 more

1 mention without citation

Structural basis for the restoration of TCR recognition of an MHC allelic variant by peptide secondary anchor substitution.
Miley et al. (2004)