› 1rl2

X-ray diffraction
2.3Å resolution

RIBOSOMAL PROTEIN L2 RNA-BINDING DOMAIN FROM BACILLUS STEAROTHERMOPHILUS

Released:
DOI: 10.2210/pdb1rl2/pdb
PDB entry 1rl2 coloured by chain, front view.
PDB entry 1rl2 coloured by chain, side view.
PDB entry 1rl2 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
The three-dimensional structure of the RNA-binding domain of ribosomal protein L2; a protein at the peptidyl transferase center of the ribosome.
Nakagawa A, Nakashima T, Taniguchi M, Hosaka H, Kimura M, Tanaka I
EMBO J 18 1459-67 (1999)
PMID: 10075918

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large ribosomal subunit protein uL2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 137 amino acids
Theoretical weight: 14.93 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04257 (Residues: 61-197; Coverage: 50%)
Gene name: rplB
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P1
Unit cell:
a: 28.05Å b: 36.19Å c: 69.71Å
α: 99.58° β: 95.86° γ: 102.63°
R-values:
R R work R free
0.203 0.203 0.258
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Ribosome assembly in eukaryotes.
Fromont-Racine et al. (2003)
3 more

8 mentions without citation

Structural Basis of IL-1 Family Cytokine Signaling.
Fields et al. (2019)
7 more