1rl3

X-ray diffraction
2.7Å resolution

Crystal structure of cAMP-free R1a subunit of PKA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-132578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 288 amino acids
Theoretical weight: 32.49 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00514 (Residues: 93-380; Coverage: 76%)
Gene name: PRKAR1A
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P65
Unit cell:
a: 90.3Å b: 90.3Å c: 177.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.24 0.285
Expression system: Escherichia coli