› 1rlz

X-ray diffraction
2.15Å resolution

Deoxyhypusine synthase holoenzyme in its high ionic strength, low pH crystal form

Released:
DOI: 10.2210/pdb1rlz/pdb
PDB entry 1rlz coloured by chain, front view.
PDB entry 1rlz coloured by chain, side view.
PDB entry 1rlz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A new crystal structure of deoxyhypusine synthase reveals the configuration of the active enzyme and of an enzyme.NAD.inhibitor ternary complex.
Umland TC, Wolff EC, Park MH, Davies DR
J Biol Chem 279 28697-705 (2004)
PMID: 15100216

Function and Biology Details

Reaction catalysed: 
(1a) spermidine + NAD(+) = dehydrospermidine + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155939 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyhypusine synthase Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 41.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49366 (Residues: 1-369; Coverage: 100%)
Gene names: DHPS, DS
Sequence domains: Deoxyhypusine synthase
Structure domains: Deoxyhypusine synthase

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P4212
Unit cell:
a: 108.6Å b: 108.6Å c: 69.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.199 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

The post-translational synthesis of a polyamine-derived amino acid, hypusine, in the eukaryotic translation initiation factor 5A (eIF5A).
Park MH. (2006)
7 more

7 mentions without citation

Hypusine, a polyamine-derived amino acid critical for eukaryotic translation.
Park et al. (2018)
6 more