› 1rmq

X-ray diffraction
2Å resolution

Crystal structure of AphA class B acid phosphatase/phosphotransferase with osmiate mimicking the catalytic intermediate

Released:
DOI: 10.2210/pdb1rmq/pdb
PDB entry 1rmq coloured by chain, front view.
PDB entry 1rmq coloured by chain, side view.
PDB entry 1rmq coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Calderone V, Forleo C, Benvenuti M, Rossolini GM, Thaller MC, Mangani S

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Class B acid phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 23.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE22 (Residues: 26-237; Coverage: 100%)
Gene names: JW4015, aphA, b4055, napA, yjbP
Sequence domains: HAD superfamily, subfamily IIIB (Acid phosphatase)
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

2 bound ligands:
Ligand CO 2 x CO
Ligand OS 4 x OS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: C2
Unit cell:
a: 91.732Å b: 66.447Å c: 91.523Å
α: 90° β: 121.3° γ: 90°
R-values:
R R work R free
0.187 0.183 0.23
Expression system: Escherichia coli

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