› 1rnb

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF A BARNASE-D(*GP*C) COMPLEX AT 1.9 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1rnb/pdb
PDB entry 1rnb coloured by chain, front view.
PDB entry 1rnb coloured by chain, side view.
PDB entry 1rnb coloured by chain, top view.
Source organism: Bacillus amyloliquefaciens
Primary publication:
Crystal structure of a barnase-d(GpC) complex at 1.9 A resolution.
Baudet S, Janin J
J Mol Biol 219 123-32 (1991)
PMID: 2023257

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114528 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Ribonuclease Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 12.4 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Not provided
UniProt:
  • Canonical: P00648 (Residues: 48-157; Coverage: 89%)
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases
DNA (5'-D(*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 2 nucleotides
Theoretical weight: 573 Da
Source organism: Bacillus amyloliquefaciens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 57.97Å b: 57.97Å c: 85.37Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 not available
Expression system: Not provided

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Citations

2 review citations

The folding of an enzyme. II. Substructure of barnase and the contribution of different interactions to protein stability.
Serrano et al. (1992)
1 more

18 mentions without citation

Protein fusion tags for efficient expression and purification of recombinant proteins in the periplasmic space of E. coli.
Malik A. (2016)
17 more