› 1rnl

X-ray diffraction
2.4Å resolution

THE NITRATE/NITRITE RESPONSE REGULATOR PROTEIN NARL FROM NARL

Released:
DOI: 10.2210/pdb1rnl/pdb
PDB entry 1rnl coloured by chain, front view.
PDB entry 1rnl coloured by chain, side view.
PDB entry 1rnl coloured by chain, top view.
Source organism: Escherichia coli str. K-12 substr. W3110
Primary publication:
Structure of the Escherichia coli response regulator NarL.
Baikalov I, Schröder I, Kaczor-Grzeskowiak M, Grzeskowiak K, Gunsalus RP, Dickerson RE
Biochemistry 35 11053-61 (1996)
PMID: 8780507

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-142691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrate/nitrite response regulator protein NarL Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 23.83 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AF28 (Residues: 2-216; Coverage: 100%)
Gene names: JW1212, b1221, frdR, narL
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PT 6 x PT
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I222
Unit cell:
a: 61.037Å b: 78.321Å c: 115.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.251
Expression system: Escherichia coli

Quick links

Citations

32 review citations

Two-component signal transduction.
Stock et al. (2000)
31 more

9 mentions without citation

Genetic evidence that transcription activation by RhaS involves specific amino acid contacts with sigma 70.
Bhende et al. (2000)
8 more