› 1ro7

X-ray diffraction
1.8Å resolution

Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analogue, CMP-3FNeuAc.

Released:
DOI: 10.2210/pdb1ro7/pdb
PDB entry 1ro7 coloured by chain, front view.
PDB entry 1ro7 coloured by chain, side view.
PDB entry 1ro7 coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog.
Chiu CP, Watts AG, Lairson LL, Gilbert M, Lim D, Wakarchuk WW, Withers SG, Strynadka NC
Nat Struct Mol Biol 11 163-70 (2004)
PMID: 14730352

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192239 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-2,3/8-sialyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 259 amino acids
Theoretical weight: 30.73 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9LAK3 (Residues: 1-259; Coverage: 89%)
Gene name: cst-II
Sequence domains: Alpha-2,3-sialyltransferase (CST-I)
Structure domains: Alpha-2,3-sialyltransferase

Ligands and Environments

2 bound ligands:
Ligand MPD 1 x MPD
Ligand CSF 4 x CSF
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 83.66Å b: 66.031Å c: 99.172Å
α: 90° β: 94.42° γ: 90°
R-values:
R R work R free
0.217 0.217 0.248
Expression system: Escherichia coli BL21

Quick links

Citations

21 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
20 more

9 mentions without citation

Sparse labeling of proteins: structural characterization from long range constraints.
Prestegard et al. (2014)
8 more