1rp0

X-ray diffraction
1.6Å resolution

Crystal Structure of Thi1 protein from Arabidopsis thaliana

Released:

Function and Biology Details

Reaction catalysed:
NAD(+) + glycine + [ADP-thiazole synthase]-L-cysteine = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H(2)O
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octamer (preferred)
PDBe Complex ID:
PDB-CPX-174364 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiamine thiazole synthase, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 30.08 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q38814 (Residues: 47-328; Coverage: 81%)
Gene names: ARA6, At5g54770, MBG8.3, THI1, THI4
Sequence domains: Thi4 family
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: F222
Unit cell:
a: 102.356Å b: 133.147Å c: 142.301Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.139 0.171
Expression system: Escherichia coli BL21(DE3)