› 1rpw

X-ray diffraction
2.9Å resolution

Crystal Structure Of The Multidrug Binding Protein Qacr Bound To The Diamidine Hexamidine

Released:
DOI: 10.2210/pdb1rpw/pdb
PDB entry 1rpw coloured by chain, front view.
PDB entry 1rpw coloured by chain, side view.
PDB entry 1rpw coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal structures of QacR-diamidine complexes reveal additional multidrug-binding modes and a novel mechanism of drug charge neutralization.
Murray DS, Schumacher MA, Brennan RG
J Biol Chem 279 14365-71 (2004)
PMID: 14726520

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator QacR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 188 amino acids
Theoretical weight: 22.15 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A0N4 (Residues: 1-188; Coverage: 100%)
Gene names: SAP100B_005, SAP104C_022, qacR
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 20 x SO4
Ligand DID 1 x DID
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P42212
Unit cell:
a: 171.8Å b: 171.8Å c: 94.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.28
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Efflux-mediated drug resistance in bacteria: an update.
Li et al. (2009)
8 more

5 mentions without citation

The TetR family of transcriptional repressors.
Ramos et al. (2005)
4 more