› 1rpy

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF THE DIMERIC SH2 DOMAIN OF APS

Released:
DOI: 10.2210/pdb1rpy/pdb
PDB entry 1rpy coloured by chain, front view.
PDB entry 1rpy coloured by chain, side view.
PDB entry 1rpy coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural basis for recruitment of the adaptor protein APS to the activated insulin receptor.
Hu J, Liu J, Ghirlando R, Saltiel AR, Hubbard SR
Mol Cell 12 1379-89 (2003)
PMID: 14690593

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-195543 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SH2B adapter protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 114 amino acids
Theoretical weight: 12.94 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Z200 (Residues: 401-510; Coverage: 18%)
Gene names: Aps, Sh2b2
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P3121
Unit cell:
a: 78.378Å b: 78.378Å c: 67.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.228 0.27
Expression system: Escherichia coli

Quick links

Citations

32 review citations

Regulation of protein kinases; controlling activity through activation segment conformation.
Nolen et al. (2004)
31 more

1 mention without citation

RASMOT-3D PRO: a 3D motif search webserver.
Debret et al. (2009)