› 1rqf

X-ray diffraction
2.89Å resolution

Structure of CK2 beta subunit crystallized in the presence of a p21WAF1 peptide

Released:
DOI: 10.2210/pdb1rqf/pdb
PDB entry 1rqf coloured by chain, front view.
PDB entry 1rqf coloured by chain, side view.
PDB entry 1rqf coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the regulatory subunit of CK2 in the presence of a p21WAF1 peptide demonstrates flexibility of the acidic loop.
Bertrand L, Sayed MF, Pei XY, Parisini E, Dhanaraj V, Bolanos-Garcia VM, Allende JE, Blundell TL
Acta Crystallogr D Biol Crystallogr 60 1698-704 (2004)
PMID: 15388915

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-219353 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Casein kinase II subunit beta Chains: A, B, D, E, G, H, J, K
Molecule details ›
Chains: A, B, D, E, G, H, J, K
Length: 184 amino acids
Theoretical weight: 21.26 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28021 (Residues: 1-178; Coverage: 83%)
Gene name: csnk2b
Sequence domains: Casein kinase II regulatory subunit
Structure domains:
Disordered segment of Cyclin-dependent kinase inhibitor 1 Chains: M, N, T
Molecule details ›
Chains: M, N, T
Length: 4 amino acids
Theoretical weight: 358 Da
Source organism: Homo sapiens
Expression system: Not provided
Disordered segment of Cyclin-dependent kinase inhibitor 1 Chain: O
Molecule details ›
Chain: O
Length: 8 amino acids
Theoretical weight: 699 Da
Source organism: Homo sapiens
Expression system: Not provided
Disordered segment of Cyclin-dependent kinase inhibitor 1 Chains: P, S
Molecule details ›
Chains: P, S
Length: 3 amino acids
Theoretical weight: 273 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand UNK 2 x UNK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 145.422Å b: 170.626Å c: 74.546Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.238 0.266
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Cellular regulators of protein kinase CK2.
Montenarh M. (2010)

1 mention without citation

Positioning of proteins in membranes: a computational approach.
Lomize et al. (2006)