› 1rs0

X-ray diffraction
2.6Å resolution

Crystal Structure Analysis of the Bb segment of Factor B complexed with Di-isopropyl-phosphate (DIP)

Released:
DOI: 10.2210/pdb1rs0/pdb
PDB entry 1rs0 coloured by chain, front view.
PDB entry 1rs0 coloured by chain, side view.
PDB entry 1rs0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase.
Ponnuraj K, Xu Y, Macon K, Moore D, Volanakis JE, Narayana SV
Mol Cell 14 17-28 (2004)
PMID: 15068800

Function and Biology Details

Reaction catalysed: 
Cleavage of Arg-|-Ser bond in complement component C3 alpha-chain to yield C3a and C3b, and Arg-|- bond in complement component C5 alpha-chain to yield C5a and C5b.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Complement factor B Bb fragment Chain: A
Molecule details ›
Chain: A
Length: 497 amino acids
Theoretical weight: 56.25 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P00751 (Residues: 268-764; Coverage: 67%)
Gene names: BF, BFD, CFB
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand IOD 2 x IOD
Ligand NA 7 x NA
Ligand MG 1 x MG
Ligand DFP 1 x DFP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P61
Unit cell:
a: 98.449Å b: 98.449Å c: 125.678Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.212 0.251
Expression system: Trichoplusia ni

Quick links

Citations

15 review citations

Complement component C3 - The "Swiss Army Knife" of innate immunity and host defense.
Ricklin et al. (2016)
14 more

3 mentions without citation

Gain-of-function mutations in complement factor B are associated with atypical hemolytic uremic syndrome.
Goicoechea de Jorge et al. (2007)
2 more