› 1rt8

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF THE ACTIN-CROSSLINKING CORE OF SCHIZOSACCHAROMYCES POMBE FIMBRIN

Released:
DOI: 10.2210/pdb1rt8/pdb
PDB entry 1rt8 coloured by chain, front view.
PDB entry 1rt8 coloured by chain, side view.
PDB entry 1rt8 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Structure of the actin crosslinking core of fimbrin.
Klein MG, Shi W, Ramagopal U, Tseng Y, Wirtz D, Kovar DR, Staiger CJ, Almo SC
Structure 12 999-1013 (2004)
PMID: 15274920

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129889 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fimbrin Chain: A
Molecule details ›
Chain: A
Length: 513 amino acids
Theoretical weight: 57.45 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O59945 (Residues: 108-614; Coverage: 83%)
Gene names: SPBC1778.06c, fim1
Sequence domains: Calponin homology (CH) domain
Structure domains: Calponin-like domain

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3121
Unit cell:
a: 84.243Å b: 84.243Å c: 150.819Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.237 0.27
Expression system: Escherichia coli BL21

Quick links

Citations

16 review citations

Actin dynamics, architecture, and mechanics in cell motility.
Blanchoin et al. (2014)
15 more

5 mentions without citation

Low ABA concentration promotes root growth and hydrotropism through relief of ABA INSENSITIVE 1-mediated inhibition of plasma membrane H+-ATPase 2.
Miao et al. (2021)
4 more