› 1rtw

X-ray diffraction
2.35Å resolution

X-ray Structure of PF1337, a TenA Homologue from Pyrococcus furiosus. Northeast Structural Genomics Research Consortium (Nesg) Target PFR34

Released:
DOI: 10.2210/pdb1rtw/pdb
PDB entry 1rtw coloured by chain, front view.
PDB entry 1rtw coloured by chain, side view.
PDB entry 1rtw coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
The 2.35 A structure of the TenA homolog from Pyrococcus furiosus supports an enzymatic function in thiamine metabolism.
Benach J, Edstrom WC, Lee I, Das K, Cooper B, Xiao R, Liu J, Rost B, Acton TB, Montelione GT, Hunt JF
Acta Crystallogr D Biol Crystallogr 61 589-98 (2005)
PMID: 15858269

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186394 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiaminase-2/PQQC domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 220 amino acids
Theoretical weight: 26.76 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U189 (Residues: 1-212; Coverage: 100%)
Gene name: PF1337
Sequence domains: TENA/THI-4/PQQC family
Structure domains: Heme oxygenase-like

Ligands and Environments

2 bound ligands:
Ligand MP5 1 x MP5
Ligand PO4 3 x PO4
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21212
Unit cell:
a: 101.8Å b: 123.65Å c: 77.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.24 0.281
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Thiamine pyrophosphate riboswitches are targets for the antimicrobial compound pyrithiamine.
Sudarsan et al. (2005)
6 more

3 mentions without citation

Structure of trifunctional THI20 from yeast.
French et al. (2011)
2 more