› 1rty

X-ray diffraction
2.4Å resolution

Crystal Structure of Bacillus subtilis YvqK, a putative ATP-binding Cobalamin Adenosyltransferase, The North East Structural Genomics Target SR128

Released:
DOI: 10.2210/pdb1rty/pdb
PDB entry 1rty coloured by chain, front view.
PDB entry 1rty coloured by chain, side view.
PDB entry 1rty coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Functional insights from structural genomics.
Forouhar F, Kuzin A, Seetharaman J, Lee I, Zhou W, Abashidze M, Chen Y, Yong W, Janjua H, Fang Y, Wang D, Cunningham K, Xiao R, Acton TB, Pichersky E, Klessig DF, Porter CW, Montelione GT, Tong L
J Struct Funct Genomics 8 37-44 (2007)
PMID: 17588214

Function and Biology Details

Reaction catalysed: 
(1a) 2 cob(II)alamin + 2 [corrinoid adenosyltransferase] = 2 [corrinoid adenosyltransferase]-cob(II)alamin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128619 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Corrinoid adenosyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 193 amino acids
Theoretical weight: 21.61 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O34899 (Residues: 1-193; Coverage: 100%)
Gene names: BSU33150, yvqK
Sequence domains: Cobalamin adenosyltransferase
Structure domains: Cobalamin adenosyltransferase-like

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P4
Unit cell:
a: 104.35Å b: 104.35Å c: 55.985Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.224 0.28
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Ethanolamine utilization in bacterial pathogens: roles and regulation.
Garsin DA. (2010)
8 more

6 mentions without citation

Structural characterization of the active site of the PduO-type ATP:Co(I)rrinoid adenosyltransferase from Lactobacillus reuteri.
St Maurice et al. (2007)
5 more