› 1run

X-ray diffraction
2.7Å resolution

CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

Released:
DOI: 10.2210/pdb1run/pdb
PDB entry 1run coloured by chain, front view.
PDB entry 1run coloured by chain, side view.
PDB entry 1run coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Aromatic hydrogen bond in sequence-specific protein DNA recognition.
Parkinson G, Gunasekera A, Vojtechovsky J, Zhang X, Kunkel TA, Berman H, Ebright RH
Nat Struct Biol 3 837-41 (1996)
PMID: 8836098

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114542 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding transcriptional dual regulator CRP Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 23.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 2-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:
DNA (5'-D(*GP*CP*GP*AP*AP*AP*AP*AP*TP*GP*TP*GP*AP*T)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 14 nucleotides
Theoretical weight: 4.35 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(*CP*TP*AP*GP*AP*TP*CP*AP*CP*AP*TP*TP*TP*TP*TP*CP*G )-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 17 nucleotides
Theoretical weight: 5.15 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CMP 2 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: C2221
Unit cell:
a: 136.93Å b: 152.8Å c: 76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Transcription activation by catabolite activator protein (CAP).
Busby et al. (1999)
2 more

25 mentions without citation

Catabolite activator protein: DNA binding and transcription activation.
Lawson et al. (2004)
24 more