› 1rw0

X-ray diffraction
2Å resolution

Crystal structure of protein yfiH from Salmonella enterica serovar Typhi, Pfam DUF152

Released:
DOI: 10.2210/pdb1rw0/pdb
PDB entry 1rw0 coloured by chain, front view.
PDB entry 1rw0 coloured by chain, side view.
PDB entry 1rw0 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhi
Entry authors: Seetharaman J, Swaminathan S, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reactions catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
2'-deoxyadenosine + H(2)O = 2'-deoxyinosine + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187165 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 26.42 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8Z4J1 (Residues: 1-243; Coverage: 100%)
Gene names: CAJ76_18160, CAJ80_21585, CAJ85_20660, CAL67_18830, CB224_16365, CC884_20785, CC907_21700, CC972_20195, D4Z37_16950, D6Q30_17935, DNM71_21600, DNV09_20510, DS261_20975, DUQ91_16785, DUV11_19740, DVG36_20995, EBC38_21100, EID90_20505, ELQ62_21715, EU445_22075, EVI00_16290, F9R11_21320, F9Y21_21355, FI137_21945, G2227_21275, G2244_21755, G3982_004072, G3V49_002822, G4I71_004348, G4L28_003661, G4P30_004446, G4Y41_003410, G4Y55_004275, G9264_004130, G9C68_004274, G9X37_004173, GDI29_21040, GND71_004182, K3U68_13180, STY2850, ZZ17_19240, pgeF, t2617, yfiH
Sequence domains: Multi-copper polyphenol oxidoreductase laccase
Structure domains: CNF1/YfiH-like putative cysteine hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P21
Unit cell:
a: 53.33Å b: 62.123Å c: 77.745Å
α: 90° β: 110.22° γ: 90°
R-values:
R R work R free
0.208 0.208 0.24
Expression system: Escherichia coli

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