› 1rwa

X-ray diffraction
1.3Å resolution

Crystal structure of Arthrobacter aurescens chondroitin AC lyase

Released:
DOI: 10.2210/pdb1rwa/pdb
PDB entry 1rwa coloured by chain, front view.
PDB entry 1rwa coloured by chain, side view.
PDB entry 1rwa coloured by chain, top view.
Source organism: Paenarthrobacter aurescens
Primary publication:
High-resolution crystal structure of Arthrobacter aurescens chondroitin AC lyase: an enzyme-substrate complex defines the catalytic mechanism.
Lunin VV, Li Y, Linhardt RJ, Miyazono H, Kyogashima M, Kaneko T, Bell AW, Cygler M
J Mol Biol 337 367-86 (2004)
PMID: 15003453

Function and Biology Details

Reaction catalysed: 
Eliminative degradation of polysaccharides containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucuronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

2 bound ligands:
Ligand HG 3 x HG
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 57.356Å b: 85.256Å c: 82.162Å
α: 90° β: 105.78° γ: 90°
R-values:
R R work R free
0.134 0.134 0.156

Quick links

Citations

10 review citations

Structural and mechanistic classification of uronic acid-containing polysaccharide lyases.
Garron et al. (2010)
9 more

3 mentions without citation

Docking server for the identification of heparin binding sites on proteins.
Mottarella et al. (2014)
2 more