› 1rxi

X-ray diffraction
1.5Å resolution

pI258 arsenate reductase (ArsC) triple mutant C10S/C15A/C82S

Released:
DOI: 10.2210/pdb1rxi/pdb
PDB entry 1rxi coloured by chain, front view.
PDB entry 1rxi coloured by chain, side view.
PDB entry 1rxi coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
The structure of a triple mutant of pI258 arsenate reductase from Staphylococcus aureus and its 5-thio-2-nitrobenzoic acid adduct.
Messens J, Van Molle I, Vanhaesebrouck P, Van Belle K, Wahni K, Martins JC, Wyns L, Loris R
Acta Crystallogr D Biol Crystallogr 60 1180-4 (2004)
PMID: 15159594

Function and Biology Details

Reaction catalysed: 
Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arsenate reductase Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.77 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A006 (Residues: 1-131; Coverage: 100%)
Gene name: arsC
Sequence domains: Low molecular weight phosphotyrosine protein phosphatase
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand K 1 x K
Ligand LCP 1 x LCP
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 32.034Å b: 34.064Å c: 63.355Å
α: 90° β: 97.328° γ: 90°
R-values:
R R work R free
0.211 0.209 0.231
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Genes and enzymes involved in bacterial oxidation and reduction of inorganic arsenic.
Silver et al. (2005)
1 more

1 mention without citation

Structural and functional mapping of ars gene cluster in Deinococcus indicus DR1.
Ranganathan et al. (2023)