› 1rxs

X-ray diffraction
2.8Å resolution

Function and Biology Details

Reaction catalysed: 
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146313 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, a, b, c, d, e, h, i, j, k, l, m, o
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, a, b, c, d, e, h, i, j, k, l, m, o
Length: 253 amino acids
Theoretical weight: 27.19 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12758 (Residues: 1-253; Coverage: 100%)
Gene names: JW3808, b3831, udp
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

4 bound ligands:
Ligand K 15 x K
Ligand V7O 20 x V7O
Ligand PO4 25 x PO4
Ligand DUR 25 x DUR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 160.958Å b: 98.212Å c: 242.729Å
α: 90° β: 109.09° γ: 90°
R-values:
R R work R free
0.178 0.176 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Role of Na+ and K+ in enzyme function.
Page et al. (2006)
21 other articles

3 mentions without citation

The use of polyoxometalates in protein crystallography - An attempt to widen a well-known bottleneck.
Bijelic et al. (2015)
2 more