1rxw

X-ray diffraction
2Å resolution

Crystal structure of A. fulgidus FEN-1 bound to DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-114551 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Flap endonuclease 1 Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 38.12 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29975 (Residues: 1-336; Coverage: 100%)
Gene names: AF_0264, fen
Sequence domains:
Structure domains:
5'-d(*C*pG*pA*pT*pG*pC*pT*pA)-3' Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.43 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided
5'-d(*T*pA*pG*pC*pA*pT*pC*pG*pG)-3' Chain: C
Molecule details ›
Chain: C
Length: 9 nucleotides
Theoretical weight: 2.76 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21212
Unit cell:
a: 109.33Å b: 86.476Å c: 43.857Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.242 0.278
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided