› 1rxy

X-ray diffraction
1.7Å resolution

Function and Biology Details

Reaction catalysed: 
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146313 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 27.19 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12758 (Residues: 1-253; Coverage: 100%)
Gene names: JW3808, b3831, udp
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

4 bound ligands:
Ligand K 1 x K
Ligand ACT 1 x ACT
Ligand SO4 1 x SO4
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: R3
Unit cell:
a: 152.928Å b: 152.928Å c: 50.864Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.13 0.128 0.164
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Role of Na+ and K+ in enzyme function.
Page et al. (2006)
1 more

1 mention without citation

Crystallization of uridine phosphorylase from Shewanella oneidensis MR-1 in the laboratory and under microgravity and preliminary X-ray diffraction analysis.
Safonova et al. (2012)