› 1ryn

X-ray diffraction
1.75Å resolution

Structure of the Chloroplast Group II Intron Splicing Factor CRS2

Released:
DOI: 10.2210/pdb1ryn/pdb
PDB entry 1ryn coloured by chain, front view.
PDB entry 1ryn coloured by chain, side view.
PDB entry 1ryn coloured by chain, top view.
Source organism: Zea mays
Primary publication:
Structural analysis of the group II intron splicing factor CRS2 yields insights into its protein and RNA interaction surfaces.
Ostheimer GJ, Hadjivassiliou H, Hadjivasiliou H, Kloer DP, Barkan A, Matthews BW
J Mol Biol 345 51-68 (2005)
PMID: 15567410

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192371 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 202 amino acids
Theoretical weight: 22.55 KDa
Source organism: Zea mays
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9M5P4 (Residues: 58-250; Coverage: 75%)
Gene name: CRS2
Sequence domains: Peptidyl-tRNA hydrolase
Structure domains: Peptidyl-tRNA hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 39.7Å b: 56.489Å c: 69.354Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.195 0.278
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Chloroplast RNA metabolism.
Stern et al. (2010)
2 more

2 mentions without citation

Structure of Francisella tularensis peptidyl-tRNA hydrolase.
Clarke et al. (2011)
1 more