› 1ryq

X-ray diffraction
1.38Å resolution

Putative DNA-directed RNA polymerase, subunit e'' from Pyrococcus Furiosus Pfu-263306-001

Released:
DOI: 10.2210/pdb1ryq/pdb
PDB entry 1ryq coloured by chain, front view.
PDB entry 1ryq coloured by chain, side view.
PDB entry 1ryq coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Parameter-space screening: a powerful tool for high-throughput crystal structure determination.
Liu ZJ, Lin D, Tempel W, Praissman JL, Rose JP, Wang BC
Acta Crystallogr D Biol Crystallogr 61 520-7 (2005)
PMID: 15858261

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription elongation factor Spt4 Chain: A
Molecule details ›
Chain: A
Length: 69 amino acids
Theoretical weight: 7.88 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U440 (Residues: 2-61; Coverage: 98%)
Gene names: PF0255, spt4
Sequence domains: Spt4/RpoE2 zinc finger
Structure domains: Rubrerythrin, domain 2

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3221
Unit cell:
a: 45.534Å b: 45.534Å c: 50.76Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.193 0.204
Expression system: Escherichia coli

Quick links

Citations

17 citation in other articles

Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7.
Langer et al. (2008)
16 more

2 mentions without citation

Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif.
Hirtreiter et al. (2010)
1 more