› 1rz2

X-ray diffraction
1.6Å resolution

1.6A crystal structure of the protein BA4783/Q81L49 (similar to sortase B) from Bacillus anthracis.

Released:
DOI: 10.2210/pdb1rz2/pdb
PDB entry 1rz2 coloured by chain, front view.
PDB entry 1rz2 coloured by chain, side view.
PDB entry 1rz2 coloured by chain, top view.
Source organism: Bacillus anthracis str. Ames
Primary publication:
Structures of sortase B from Staphylococcus aureus and Bacillus anthracis reveal catalytic amino acid triad in the active site.
Zhang R, Wu R, Joachimiak G, Mazmanian SK, Missiakas DM, Gornicki P, Schneewind O, Joachimiak A
Structure 12 1147-56 (2004)
PMID: 15242591

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105773 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SrtB family sortase Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 30.14 KDa
Source organism: Bacillus anthracis str. Ames
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A6L8PZR0 (Residues: 1-254; Coverage: 100%)
Gene name: GBAA_4783
Sequence domains: Sortase domain
Structure domains: Sortase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 40.474Å b: 64.599Å c: 42.964Å
α: 90° β: 105.77° γ: 90°
R-values:
R R work R free
0.225 0.225 0.266
Expression system: Escherichia coli BL21

Quick links

Citations

17 review citations

Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria.
Marraffini et al. (2006)
16 more

7 mentions without citation

Sortase Transpeptidases: Structural Biology and Catalytic Mechanism.
Jacobitz et al. (2017)
6 more