› 1rzi

X-ray diffraction
2.9Å resolution

Crystal structure of human anti-HIV-1 gp120-reactive antibody 47e fab

Released:
DOI: 10.2210/pdb1rzi/pdb
PDB entry 1rzi coloured by chain, front view.
PDB entry 1rzi coloured by chain, side view.
PDB entry 1rzi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of tyrosine sulfation and VH-gene usage in antibodies that recognize the HIV type 1 coreceptor-binding site on gp120.
Huang CC, Venturi M, Majeed S, Moore MJ, Phogat S, Zhang MY, Dimitrov DS, Hendrickson WA, Robinson J, Sodroski J, Wyatt R, Choe H, Farzan M, Kwong PD
Proc Natl Acad Sci U S A 101 2706-11 (2004)
PMID: 14981267

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213085 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fab 47e light chain Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 212 amino acids
Theoretical weight: 23.17 KDa
Source organism: Homo sapiens
Expression system: Human gammaherpesvirus 4
Structure domains: Immunoglobulins
Fab 47e heavy chain Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 230 amino acids
Theoretical weight: 24.16 KDa
Source organism: Homo sapiens
Expression system: Human gammaherpesvirus 4
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 73.43Å b: 111.645Å c: 133.309Å
α: 85.48° β: 90° γ: 89.71°
R-values:
R R work R free
0.233 0.233 0.321
Expression system: Human gammaherpesvirus 4

Quick links

Citations

37 review citations

Antibody vs. HIV in a clash of evolutionary titans.
Burton et al. (2005)
36 more

10 mentions without citation

Structural basis of tyrosine sulfation and VH-gene usage in antibodies that recognize the HIV type 1 coreceptor-binding site on gp120.
Huang et al. (2004)
9 more