› 1rzn

X-ray diffraction
2.3Å resolution

Crystal Structure of Penicillin-binding protein-related factor A from Bacillus Subtilis.

Released:
DOI: 10.2210/pdb1rzn/pdb
PDB entry 1rzn coloured by chain, front view.
PDB entry 1rzn coloured by chain, side view.
PDB entry 1rzn coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Ramagopal UA, Almo SC, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Holliday junction resolvase RecU Chains: A, B
Molecule details ›
Chains: A, B
Length: 205 amino acids
Theoretical weight: 23.86 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P39792 (Residues: 2-206; Coverage: 100%)
Gene names: BSU22310, prfA, recU, yppB
Sequence domains: Recombination protein U
Structure domains: Trna Endonuclease; Chain: A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P65
Unit cell:
a: 74.303Å b: 74.303Å c: 155.81Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.186 0.245
Expression system: Escherichia coli

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