› 1rzy

X-ray diffraction
1.8Å resolution

Crystal structure of rabbit Hint complexed with N-ethylsulfamoyladenosine

Released:
DOI: 10.2210/pdb1rzy/pdb
PDB entry 1rzy coloured by chain, front view.
PDB entry 1rzy coloured by chain, side view.
PDB entry 1rzy coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Biochemical, crystallographic, and mutagenic characterization of hint, the AMP-lysine hydrolase, with novel substrates and inhibitors.
Krakowiak A, Pace HC, Blackburn GM, Adams M, Mekhalfia A, Kaczmarek R, Baraniak J, Stec WJ, Brenner C
J Biol Chem 279 18711-6 (2004)
PMID: 14982931

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine 5'-monophosphoramidase HINT1 Chain: A
Molecule details ›
Chain: A
Length: 126 amino acids
Theoretical weight: 13.71 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P80912 (Residues: 2-126; Coverage: 99%)
Gene names: HINT, HINT1
Sequence domains: HIT domain
Structure domains: HIT-like

Ligands and Environments

1 bound ligand:
Ligand 5AS 1 x 5AS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 39.888Å b: 39.888Å c: 142.286Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.216 0.242
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Hit proteins, mitochondria and cancer.
Martin et al. (2011)
4 more

2 mentions without citation

Biochemical, crystallographic, and mutagenic characterization of hint, the AMP-lysine hydrolase, with novel substrates and inhibitors.
Krakowiak et al. (2004)
1 more