› 1s03

X-ray diffraction
2.7Å resolution

The Structure of a Ribosomal Protein S8/spc Operon mRNA Complex

Released:
DOI: 10.2210/pdb1s03/pdb
PDB entry 1s03 coloured by chain, front view.
PDB entry 1s03 coloured by chain, side view.
PDB entry 1s03 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structure of a ribosomal protein S8/spc operon mRNA complex.
Merianos HJ, Wang J, Moore PB
RNA 10 954-64 (2004)
PMID: 15146079

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141631 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Small ribosomal subunit protein uS8 Chains: G, H
Molecule details ›
Chains: G, H
Length: 129 amino acids
Theoretical weight: 14.02 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W7 (Residues: 2-130; Coverage: 99%)
Gene names: JW3268, b3306, rpsH
Sequence domains: Ribosomal protein S8
Structure domains:
47-MER Chains: A, B
Molecule details ›
Chains: A, B
Length: 47 nucleotides
Theoretical weight: 15.12 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 17 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3221
Unit cell:
a: 61.22Å b: 61.22Å c: 332.52Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.237 0.273
Expression system: Not provided

Quick links

Citations

8 review citations

Regulation of translation via mRNA structure in prokaryotes and eukaryotes.
Kozak M. (2005)
7 more

15 mentions without citation

Computational approaches to 3D modeling of RNA.
Laing et al. (2010)
14 more