› 1s0v

X-ray diffraction
3.2Å resolution

Structural basis for substrate selection by T7 RNA polymerase

Released:
DOI: 10.2210/pdb1s0v/pdb
PDB entry 1s0v coloured by chain, front view.
PDB entry 1s0v coloured by chain, side view.
PDB entry 1s0v coloured by chain, top view.
Source organism: Escherichia phage T7
Primary publication:
Structural basis for substrate selection by t7 RNA polymerase.
Temiakov D, Patlan V, Anikin M, McAllister WT, Yokoyama S, Vassylyev DG
Cell 116 381-91 (2004)
PMID: 15016373

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114562 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
T7 RNA polymerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 883 amino acids
Theoretical weight: 98.98 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00573 (Residues: 1-496, 498-883; Coverage: 100%)
Gene name: 1
Sequence domains:
Structure domains:
5'-D(*G*GP*GP*AP*AP*TP*CP*GP*AP*TP*AP*TP*CP*GP*CP*CP*GP*C)-3' Chains: E, H, K, N
Molecule details ›
Chains: E, H, K, N
Length: 18 nucleotides
Theoretical weight: 5.54 KDa
5'-D(*GP*TP*CP*GP*AP*TP*TP*CP*CP*C)-3' Chains: G, J, M, P
Molecule details ›
Chains: G, J, M, P
Length: 10 nucleotides
Theoretical weight: 3 KDa
5'-R(*AP*AP*CP*U*GP*CP*GP*GP*CP*GP*AP*U)-3' Chains: F, I, L, O
Molecule details ›
Chains: F, I, L, O
Length: 12 nucleotides
Theoretical weight: 3.85 KDa

Ligands and Environments

2 bound ligands:
Ligand MG 8 x MG
Ligand APC 4 x APC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8
Spacegroup: P1
Unit cell:
a: 81.133Å b: 87.703Å c: 206.531Å
α: 91.93° β: 91.02° γ: 110.66°
R-values:
R R work R free
0.255 0.255 0.307
Expression system: Escherichia coli

Quick links

Citations

14 review citations

RNA polymerase fidelity and transcriptional proofreading.
Sydow et al. (2009)
13 more

12 mentions without citation

Computational simulation strategies for analysis of multisubunit RNA polymerases.
Wang et al. (2013)
11 more